Generator

Part:BBa_K2796056:Design

Designed by: Tuoyu Zhou   Group: iGEM18_LZU-CHINA   (2018-10-07)


pTRE-(hsa-miR-942-5p)-WPRE-SV40 poly(A) signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal AgeI site found at 271
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We need to follow the RFC 10 rule and consider how to use lentiviral vectors to enhance gene expression in cells.So we introduced the WPRE component.


Source

NCBI

References